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For subcutaneous injection, you want the needle to reach the fat layer beneath the skin without penetrating into the muscle below

Also, a similar protocol was successful in the therapy of the other fistulas, that is, gastrocutaneous (Skorjanec et al., 2009), esophagocutaneous (Skorjanec et al., 2015), vesicovaginal (Seiwerth et al., 1997

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Once the injection has been administered, pressure will be applied to the area

Enhanced microcirculation is thought to facilitate delivery of metabolic substrates and signaling molecules necessary for connective tissue repair